asisi (New England Biolabs)
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asisi
Asisi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asisi/AsiSI/pmc12969024-258-21-22
Average 96 stars, based on 255 article reviews
Asisi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asisi/AsiSI/pmc12969024-258-21-22
Average 96 stars, based on 255 article reviews
asisi - by Bioz Stars,
2026-09
96/100 stars
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Expressing:Article Title: Hierarchical TBX6-FOXC Regulatory Logic Controls Human Trunk Mesoderm Diversification Article Snippet: .. For Dox-inducible expression of transgenes of interest, the dCas9-KRAB-U6-gRNA cassette was excised with NotI (NEB, R3189) and Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. Clone Assay:Article Title: Hierarchical TBX6-FOXC Regulatory Logic Controls Human Trunk Mesoderm Diversification Article Snippet: .. For Dox-inducible expression of transgenes of interest, the dCas9-KRAB-U6-gRNA cassette was excised with NotI (NEB, R3189) and Article Title: Targeted Covalent Inhibition of Plasmodium FK506 Binding Protein 35 Article Snippet: .. For NanoBRET assays, full length Plasmodium falciparum FKBP35 and human FKBP12 DNA were ordered as gBlocks from IDT and cloned using the Promega Flexi® system (Promega, cat. C8640). into NanoLuc® Luciferase Reporter pFN31 vectors (Promega, cat. N1311) with other:Article Title: Characterization of variants associated with Cerebral Small Vessel Disease identifies a functional SNV in Versican Article Snippet: 10μg of pMPRAv3:Δluc:ΔxbaI (Addgene) was digested with Article Title: Single-cell CRISPR activation screens in primary B cells discover gene regulatory mechanisms for hundreds of autoimmune risk loci Article Snippet: To clone the MPRA library into lentiviral vector pLS-mP (a gift from Nadav Ahituv; Addgene #81225), 125 ng of the oligonucleotide pool DNA was amplified in 4 × 50 μL reactions with 0.5 μM MPRAlib_PCR1_FW and MPRAlib_PCR1_RV primers and following conditions: 98°C for 1 min, (98°C for 10 s, 60°C for 15 s, 72°C for 20 s) × 5 cycles, 72°C for 5 min. 150 ng of purified PCR product split into 4 × 50 μL reactions and amplified with 0.5 μM MPRAlib_PCR2_FW and MPRAlib_BC_PCR2_RV primers under following conditions: 98°C for 2 min, (98°C for 15 s, 60°C for 20 s, 72°C for 30 s) × 4 cycles, 72°C for 5 min. At this step, a unique 13 bp molecular barcode (from primer MPRA_PCR2_FW) was added to each CRE. Control:Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and Article Title: Intravenous Oncolytic Vaccinia Expressing Transgenes for Enhanced Safety, Inhibition of Shedding and Systemic Cancer Immunotherapy Article Snippet: 393 All the modifications in the viruses described in Table 1 were generated using the same 394 procedure by recombination and reactivation of vaccinia virus DNA.39 Codon optimized 395 transgene sequences of wild type (WT) HSV-TK and HSV-TK variants TK.007, SR39, and 396 dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking 397 regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 398 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified40 and 399 digested with restriction enzymes AscI (NEB, Ipswich, MA, R0558) and Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. Virus:Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and Article Title: Intravenous Oncolytic Vaccinia Expressing Transgenes for Enhanced Safety, Inhibition of Shedding and Systemic Cancer Immunotherapy Article Snippet: 393 All the modifications in the viruses described in Table 1 were generated using the same 394 procedure by recombination and reactivation of vaccinia virus DNA.39 Codon optimized 395 transgene sequences of wild type (WT) HSV-TK and HSV-TK variants TK.007, SR39, and 396 dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking 397 regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 398 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified40 and 399 digested with restriction enzymes AscI (NEB, Ipswich, MA, R0558) and Purification:Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy Article Snippet: Codon-optimized transgene sequences of WT HSV-TK and HSV-TK variants TK.007, SR39, and dm30 were synthetized with the P-SE/L synthetic early-late promoter and homologous flanking regions for the ΔJ2R insertion site (GenScript, Piscataway, NJ) and liberated from the pUC57 plasmid via PCR amplification. .. Viral genomic DNA from the control virus was purified and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and Plasmid Preparation:Article Title: The role of the lysine histone methylase KMT2D in chronic myeloid leukemia Article Snippet: The KMT2D -encoding plasmid was provided by Promega (Cat# FHC12732, Madison, Wisconsin, United States) and the plasmid harboring the KMT2D p. (Trp191Arg) variant was obtained by mutagenesis at GenScript (Rijswijk, Netherlands). .. The empty pFN21A vector was obtained through restriction enzyme cloning with Cloning:Article Title: The role of the lysine histone methylase KMT2D in chronic myeloid leukemia Article Snippet: The KMT2D -encoding plasmid was provided by Promega (Cat# FHC12732, Madison, Wisconsin, United States) and the plasmid harboring the KMT2D p. (Trp191Arg) variant was obtained by mutagenesis at GenScript (Rijswijk, Netherlands). .. The empty pFN21A vector was obtained through restriction enzyme cloning with Sequencing:Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. Polymerase Chain Reaction:Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. FLAG-tag:Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. Construct:Article Title: Novel missense variants in COX15 cause oocyte degeneration and female infertility. Article Snippet: Purpose To identify novel pathogenic genes and variants responsible for oocyte degeneration and death.. Methods Whole-exome sequencing was conducted in 78 individuals with primary infertility characterized with oocyte degeneration and death, followed by Sanger sequencing of candidate variants.. Pathogenicity of identified COX15 variants was characterized through morphological assessment, AlphaFold3-based structural modeling and functional validation including Western blotting and immunofluorescence. Luciferase:Article Title: Targeted Covalent Inhibition of Plasmodium FK506 Binding Protein 35 Article Snippet: .. For NanoBRET assays, full length Plasmodium falciparum FKBP35 and human FKBP12 DNA were ordered as gBlocks from IDT and cloned using the Promega Flexi® system (Promega, cat. C8640). into NanoLuc® Luciferase Reporter pFN31 vectors (Promega, cat. N1311) with |
